AbD Serotec/Tocilizumab antibody | AbD21362/0.1 mg/HCA252
商品编号：
HCA252
品牌：
AbDSerotec
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆基因
公司分类：
Cloned_Genes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Human Anti-Tocilizumab Antibody, clone AbD21362 is a paratope specific, anti-idiotypic antibody that binds to free tocilizumab but not to human Interleukin-6 Receptor (IL-6R) or the drug/IL-6R complex. The antibody can be used to measure the levels of tocilizumab and biosimilar products in bioanalytical and patient monitoring assays.A pair of anti-tocilizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as a capture antibody, paired with an HRP conjugated Anti-Tocilizumab Antibody in full immunoglobulin format, clone AbD21338_hIgG1 (HCA253) as the detection antibody.Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.View a summary of all anti-tocilizumab antibodies
Product Details
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Tocilizumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.9 nM by real time, label-free molecular interaction analysis on immobilized tocilizumab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Tocilizumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 |
- 1 NB. The Rat anti DYKDDDDK-tag antibody, clone 6F7 is not suitable for use with this antibody as clone 6F7 exhibits cross reactivity with the human IL-6R
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct or indirect ELISA or as a capture reagent in a PK bridging ELISA together with HCA253 as the detection reagent. Protocol: PK bridging ELISA to measure free drug
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |  |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Tocilizumab | HCA253 | E | 0.1 mg | | |
| Human anti Tocilizumab | HCA254 | E | 0.1 mg | | |
| Human anti Tocilizumab | HCA255 | E | 0.1 mg | | |
| Human anti Tocilizumab | HCA256 | E | 0.1 mg | | |
| Human anti Tocilizumab | HCA257 | E | 0.1 mg | | |
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
View more products with Tocilizumab specificity
品牌介绍
AbD Serotec一抗以高特异性和亲和力直接结合特异性抗原。它们可以是与特定表位结合的单克隆抗体，也可以是与抗原的几个表位结合的多克隆抗体。一抗主要用于免疫测定，例如ELISA，蛋白质印迹，免疫组织化学，染色质免疫沉淀（ChIP）或流式细胞仪。对于抗原检测，可以将一抗结合到报告酶或荧光团上，这有助于定量或分析抗原表达。共轭一抗体通常消除了对二抗体的需求 ; 但是某些实验条件可能需要使用它们。针对纯化的一抗的Fc部分开发了二抗，通常将二抗结合用于抗原检测。另外，它们针对第一抗体的宿主物种而开发，使得在山羊中产生的第一抗体将需要在除山羊以外的物种中产生的抗山羊第二抗体。多克隆抗体由免疫球蛋白分子的混合池组成，这些免疫球蛋白分子与单个抗原上的几种不同表位结合。多克隆抗体通常在兔，驴，绵羊和山羊中产生，并从血清中纯化。相反，单克隆抗体结合靶抗原内的单个表位。
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