AbD-Serotec/Cetuximab抗体| AbD19834/0.1mg/HCA220
商品编号：
HCA220
品牌：
AbDSerotec
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆试剂盒
公司分类：
Cloning_kit
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Human Anti-Cetuximab Antibody, clone AbD19834, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target, epidermal growth factor receptor (EGFR). It can be used in bioanalytical assays to measure the levels of cetuximab and biosimilar products in preclinical research and clinical trials.Clone AbD19834 is recommended as the capture antibody in a pharmacokinetic (PK) bridging ELISA. When paired with another inhibitory antibody, HRP conjugated Anti-Cetuximab Antibody clone AbD19830_hIgG1 (HCA221P), as detection antibody, free drug can be measured. When paired with the non-inhibitory Anti-Cetuximab Antibody, clone AbD19376_hIgG1 (HCA228P), free and partially bound drug can be measured, which is equivalent to total drug when trough serum levels are above 4 μg/ml.Cetuximab (brand name Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.View a summary of all anti cetuximab antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Cetuximab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD = 0.5 nM by real time, label free molecular interaction analysis on immobilized cetuximab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Erbitux® is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Cetuximab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a coating antibody in PK bridging assays together with HRP-conjugated HCA221 or HCA228 as the detection reagent. Protocol: PK bridging ELISA to measure free drug
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |  | |
| Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg | | |
| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg | | |
| Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg | | |
| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E PWB | 0.1 mg | | |
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl | | |
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml | |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Cetuximab | HCA221 | E | 0.1 mg | | |
| Human anti Cetuximab | HCA228 | E | 0.1 mg | | |
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg | |
Product Specific References
Further Reading
- Gomez, D. et al. (2013) Cetuximab therapy in the treatment of metastatic colorectal cancer: the future frontier?Int J Surg. 11 (7): 507-13.
View more products with Cetuximab specificity
品牌介绍
AbD Serotec一抗以高特异性和亲和力直接结合特异性抗原。它们可以是与特定表位结合的单克隆抗体，也可以是与抗原的几个表位结合的多克隆抗体。一抗主要用于免疫测定，例如ELISA，蛋白质印迹，免疫组织化学，染色质免疫沉淀（ChIP）或流式细胞仪。对于抗原检测，可以将一抗结合到报告酶或荧光团上，这有助于定量或分析抗原表达。共轭一抗体通常消除了对二抗体的需求 ; 但是某些实验条件可能需要使用它们。针对纯化的一抗的Fc部分开发了二抗，通常将二抗结合用于抗原检测。另外，它们针对第一抗体的宿主物种而开发，使得在山羊中产生的第一抗体将需要在除山羊以外的物种中产生的抗山羊第二抗体。多克隆抗体由免疫球蛋白分子的混合池组成，这些免疫球蛋白分子与单个抗原上的几种不同表位结合。多克隆抗体通常在兔，驴，绵羊和山羊中产生，并从血清中纯化。相反，单克隆抗体结合靶抗原内的单个表位。
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