AbD-Serotec/IL-12/IL-23抗体| CC326/0.25 mg/MCA2173Z
商品编号：
MCA2173Z
品牌：
AbDSerotec
市场价：
¥0.00
美元价：
0.00
产品分类：
克隆基因
公司分类：
Cloned_Genes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 recognizes the p40 subunit of bovine interleukin-12. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 has been shown to block the biological activity of bovine IL-12.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant bovine IL-12.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46282
- P54349
- Entrez Gene
- IL12B
- IL12A
- GO Terms
- GO:0016020membrane
- GO:0004896cytokine receptor activity
- GO:0005125cytokine activity
- GO:0005143interleukin-12 receptor binding
- GO:0030101natural killer cell activation
- GO:0042093T-helper cell differentiation
- GO:0042095interferon-gamma biosynthetic process
- GO:0042104positive regulation of activated T cell proliferation
- GO:0008083growth factor activity
- Regulatory
- For research purposes only
Applications of IL-12 / IL-23 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/1000 | |
| Flow Cytometry 1 | 1/1000 | ||
| Functional Assays | 1/100 | ||
| ELISA | 5ug/ml |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells of 100ul.
- ELISA
- This biotin conjugate may be used as detection reagent in a sandwich ELISA assay using MCA1782EL as capture reagent, see Bannerman, D.D.et al.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E WB | 0.5 mg |  | |
| Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) | STAR117D488GA | F IF | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) | STAR117D680GA | F WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg | | |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg | | |
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg | | |
| Goat anti Mouse IgG (Fc):FITC | STAR120F | C F | 1 mg | | |
| Goat anti Mouse IgG (Fc):HRP | STAR120P | E WB | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml | | |
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg | | |
| Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg | | |
| Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml | | |
| Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg | | |
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg | | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:Dylight®800 | STAR8D800GA | F IF WB | 0.1 mg | | |
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg | |
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control:Preservative Free | MCA691XZ | C F P R RE | 1 mg | |
Application Based External Images
ELISA
Flow Cytometry
Product Specific References
References for IL-12 / IL-23 antibody
- Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.J Immunol Methods. 266 (1-2): 117-26.
- Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70.
- Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.Funct Integr Genomics. 10: 21-38.
- Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19.
- Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.J Immunol. 185: 3313-25.
- Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.Clin Diagn Lab Immunol. 11: 463-72.
- Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705.
- Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.J Virol. 82: 1777-86.
- Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.Immunology. 110: 48-57.
- Verhelst, D. et al. (2014) Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res.10: 293.
- Beechler, B.R. et al. (2015) Enemies and turncoats: bovine tuberculosis exposes pathogenic potential of Rift Valley fever virus in a common host, African buffalo (Syncerus caffer).Proc Biol Sci. 282 (1805) pii: 20142942.
- Rutigliano, H.M. et al. (2016) Trophoblast Major Histocompatibility Complex Class I Expression Is Associated with Immune-Mediated Rejection of Bovine Fetuses Produced by Cloning.Biol Reprod. 95 (2): 39.
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品牌介绍
AbD Serotec一抗以高特异性和亲和力直接结合特异性抗原。它们可以是与特定表位结合的单克隆抗体，也可以是与抗原的几个表位结合的多克隆抗体。一抗主要用于免疫测定，例如ELISA，蛋白质印迹，免疫组织化学，染色质免疫沉淀（ChIP）或流式细胞仪。对于抗原检测，可以将一抗结合到报告酶或荧光团上，这有助于定量或分析抗原表达。共轭一抗体通常消除了对二抗体的需求 ; 但是某些实验条件可能需要使用它们。针对纯化的一抗的Fc部分开发了二抗，通常将二抗结合用于抗原检测。另外，它们针对第一抗体的宿主物种而开发，使得在山羊中产生的第一抗体将需要在除山羊以外的物种中产生的抗山羊第二抗体。多克隆抗体由免疫球蛋白分子的混合池组成，这些免疫球蛋白分子与单个抗原上的几种不同表位结合。多克隆抗体通常在兔，驴，绵羊和山羊中产生，并从血清中纯化。相反，单克隆抗体结合靶抗原内的单个表位。
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