AbD-Serotec/TNF-α抗体| CC328/0.25 mg/MCA2335B
商品编号：
MCA2335B
品牌：
AbDSerotec
市场价：
¥0.00
美元价：
0.00
产品分类：
转录表达
公司分类：
Target_Expression
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Mouse anti Bovine TNF alpha antibody, clone CC328 recognizes bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Recombinant bovine TNF alpha.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q06599
- Entrez Gene
- TNF
- GO Terms
- GO:0005125cytokine activity
- GO:0005615extracellular space
- Regulatory
- For research purposes only
Applications of TNF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- Biotinylated Mouse anti Bovine TNF alpha antibody, clone CC328 may be used as a detection antibody in a sandwich ELISA for bovine TNF alpha in combination with Mouse anti Bovine TNFα antibody, clone CC327 (MCA2334) as capture reagent. Recombinant Bovine TNFα (PBP005) may be used as a standard.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Application Based External Images
ELISA
Product Specific References
References for TNF Alpha antibody
- Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides.Vet. Immunol. Immunopathol. 95: 21-31.
- Whelan, A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide.Infect Immun. 71 (11): 6420-5.
- Guergnon J et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells.Cell Microbiol. 5 (10): 709-16.
- Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha.Vet Immunol Immunopathol. 135 (3-4): 320-4.
- Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.Am J Physiol Lung Cell Mol Physiol. 300 (1): L12-24.
- Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.Vet Res. 42: 49.
- Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.J Comp Pathol. 150 (4): 434-48.
- Camejo, M.I. et al. (2014) TNF-alpha in bulls experimentally infected with Trypanosoma vivax: a pilot study.Vet Immunol Immunopathol. 162 (3-4): 192-7.
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View more products with TNF ALPHA specificity
品牌介绍
AbD Serotec一抗以高特异性和亲和力直接结合特异性抗原。它们可以是与特定表位结合的单克隆抗体，也可以是与抗原的几个表位结合的多克隆抗体。一抗主要用于免疫测定，例如ELISA，蛋白质印迹，免疫组织化学，染色质免疫沉淀（ChIP）或流式细胞仪。对于抗原检测，可以将一抗结合到报告酶或荧光团上，这有助于定量或分析抗原表达。共轭一抗体通常消除了对二抗体的需求 ; 但是某些实验条件可能需要使用它们。针对纯化的一抗的Fc部分开发了二抗，通常将二抗结合用于抗原检测。另外，它们针对第一抗体的宿主物种而开发，使得在山羊中产生的第一抗体将需要在除山羊以外的物种中产生的抗山羊第二抗体。多克隆抗体由免疫球蛋白分子的混合池组成，这些免疫球蛋白分子与单个抗原上的几种不同表位结合。多克隆抗体通常在兔，驴，绵羊和山羊中产生，并从血清中纯化。相反，单克隆抗体结合靶抗原内的单个表位。
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