AbD-Serotec/Tocilizumab抗体| AbD 22155_hIgG1/0.1 mg/HCA257P-免疫在线蚂蚁淘旗下平台

商品详细AbD-Serotec/Tocilizumab抗体| AbD 22155_hIgG1/0.1 mg/HCA257P

AbD-Serotec/Tocilizumab抗体| AbD 22155_hIgG1/0.1 mg/HCA257P

商品编号： HCA257P

品牌： AbDSerotec

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：克隆基因

公司分类： Cloned_Genes

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Summary
Useful Reagents
References (1)

Human anti tocilizumab, clone AbD22155_hIgG1 is a non-paratope specific, anti-idiotypic antibody that binds to free tocilizumab and also to tocilizumab in bound to its target, human interleukin-6 receptor (IL-6R). The antibody can be used to measure total levels of tocilizumab and biosimilar products in bioanalytical assays.Clone AbD22155_hIgG1 can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug levels (free drug, partially bound and fully bound). The antibody, HRP-conjugated and in full immunoglobulin (Ig) format, is recommended as the detection antibody, paired with capture antibody clone AbD21362 (HCA252) in monovalent Fab format. The antibody in Ig format can also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera.Tocilizumab, marketed under the brand name RoActemra^® in Europe and Actemra^® in North America, is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody, directed against IL-6R, acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.View a summary of all anti-tocilizumab antibodies

Product Details

Product Form: Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Product Form: Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Tocilizumab and tocilizumab-sIL-6R complex
Affinity: The monovalent intrinsic affinity of this antibody was measured as K_D = 31 nM by real time, label free molecular interaction analysis on immobilized tocilizumab.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at -70^oC.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage: Store at +4^oC or at -20^oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory: For research purposes only

Applications of Tocilizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in theHuCAL Antibodies Technical Manual
ELISA: This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.Protocol: PK bridging ELISA to measure total drug
ELISA: This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.Protocol: PK bridging ELISA to measure total drug

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Hispec Assay Diluent	BUF049A	E IY	50 ml	Log in	Get Quote
Human anti Tocilizumab	HCA252	E*	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA253	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA254	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA255	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA256	E	0.1 mg	Log in	Get Quote
Hispec Assay Diluent	BUF049A	E IY	50 ml	Log in	Get Quote
Human anti Tocilizumab	HCA252	E*	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA253	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA254	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA255	E	0.1 mg	Log in	Get Quote
Human anti Tocilizumab	HCA256	E	0.1 mg	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK001P	CJ	1 Conjugation For 400µg Antibody	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK002P	CJ	3 Conjugations For 400µg Antibody	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK003P	CJ	1 Conjugation For 4mg Antibody	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK004P	CJ	5 Conjugations For 4mg Antibody	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK005P	CJ	1 Conjugation For 20mg Antibody	Log in	Get Quote
LYNX Rapid HRP Antibody Conjugation Kit	LNK006P	CJ	3 Conjugations For 40µg Antibody	Log in	Get Quote

Product Specific References

References for Tocilizumab antibody

Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibodyUS Patent Application US20170315118A1

View more products with Tocilizumab specificity

品牌介绍

AbD Serotec一抗以高特异性和亲和力直接结合特异性抗原。它们可以是与特定表位结合的单克隆抗体，也可以是与抗原的几个表位结合的多克隆抗体。一抗主要用于免疫测定，例如ELISA，蛋白质印迹，免疫组织化学，染色质免疫沉淀（ChIP）或流式细胞仪。对于抗原检测，可以将一抗结合到报告酶或荧光团上，这有助于定量或分析抗原表达。共轭一抗体通常消除了对二抗体的需求 ; 但是某些实验条件可能需要使用它们。针对纯化的一抗的Fc部分开发了二抗，通常将二抗结合用于抗原检测。另外，它们针对第一抗体的宿主物种而开发，使得在山羊中产生的第一抗体将需要在除山羊以外的物种中产生的抗山羊第二抗体。多克隆抗体由免疫球蛋白分子的混合池组成，这些免疫球蛋白分子与单个抗原上的几种不同表位结合。多克隆抗体通常在兔，驴，绵羊和山羊中产生，并从血清中纯化。相反，单克隆抗体结合靶抗原内的单个表位。